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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
08/02/2022 |
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Data da última atualização: |
08/02/2022 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVEIRA, J. G. da; ASSAD, L. T.; OLIVEIRA NETO, S. N. de; BONET, M. S.; LEITE, F. F. G. D.; CANTO, A. C. B. do; CORDEIRO, F. R.; MUÑOZ, A. M.; RAMOS, M. B. de C.; RODRIGUES, R. de A. R. |
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Afiliação: |
JULIA GRAZIELA DA SILVEIRA, UFV; LUÍS TADEU ASSAD, IABS; SÍLVIO NOLASCO DE OLIVEIRA NETO, UFV; MARÍA SUÁREZ BONET, IABS; FERNANDA FIGUEIREDO GRANJA DORILÊO LEITE, UFF; ANA CAROLINA BARBOSA DO CANTO, UFF; FERNANDA REIS CORDEIRO, UFF; ALEJANDRO MUÑOZ MUÑOZ, IABS; MARÍLIA BEATRIZ DE CASTRO RAMOS, IABS; RENATO DE ARAGAO RIBEIRO RODRIGUES, CNPS. |
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Título: |
Selection of species for implantation in low-carbon technology in the Brazilian Atlantic Forest. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. WCCLF 2021 proceedings. Brasília, DF: Embrapa, 2021. p. 738-743. WCCLF 2021. Evento online. |
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Idioma: |
Inglês |
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Conteúdo: |
The Atlantic Forest has a history of agricultural occupation and expansion that changed the original ecosystem, increase loss of native vegetation and degraded areas. By adopting low-carbon technologies, producers can promote food security and contribute to mitigate climate change. This type of technology is practicable through projects such as Sustainable Rural. The success of this type of project is related to the appropriate choice of species for different environmental conditions. Thus, the objective of this work was to identify, from trained specialists and rural producers, the main species for implantation of different low-carbon technologies in the Atlantic Forest, being: Agroforestry Systems (AFS), Recovery of Degraded Area with Pasture (RDAP), Recovery of Degraded Area with Forest (RDAF), Forest Planting (FP) and Management of Native Forest (MNF). 2,020 properties were supported with the technologies, and 376 species of interest were identified. The RDAF and MNF had the greatest diversity of species group. AFS and RDAP concentrated on one main species, Theobroma cacao and Avena spp. respectively. Thus, it is found a listing of species with potential for projects with different low-carbon technologies in the Atlantic Forest, which are adapted to the local market and with economic and environmental benefits. |
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Palavras-Chave: |
Sustainability. |
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Thesagro: |
Desenvolvimento Rural; Mudança Climática. |
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Thesaurus Nal: |
Climate change; Rural development; Sustainable development. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/231057/1/Selection-of-species-for-implantation-in-low-carbon-technology-2021.pdf
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Marc: |
LEADER 02351nam a2200289 a 4500
001 2139798
005 2022-02-08
008 2021 bl uuuu u00u1 u #d
100 1 $aSILVEIRA, J. G. da
245 $aSelection of species for implantation in low-carbon technology in the Brazilian Atlantic Forest.$h[electronic resource]
260 $aIn: WORLD CONGRESS ON INTEGRATED CROP-LIVESTOCK-FORESTRY SYSTEMS, 2., 2021. WCCLF 2021 proceedings. Brasília, DF: Embrapa, 2021. p. 738-743. WCCLF 2021. Evento online.$c2021
520 $aThe Atlantic Forest has a history of agricultural occupation and expansion that changed the original ecosystem, increase loss of native vegetation and degraded areas. By adopting low-carbon technologies, producers can promote food security and contribute to mitigate climate change. This type of technology is practicable through projects such as Sustainable Rural. The success of this type of project is related to the appropriate choice of species for different environmental conditions. Thus, the objective of this work was to identify, from trained specialists and rural producers, the main species for implantation of different low-carbon technologies in the Atlantic Forest, being: Agroforestry Systems (AFS), Recovery of Degraded Area with Pasture (RDAP), Recovery of Degraded Area with Forest (RDAF), Forest Planting (FP) and Management of Native Forest (MNF). 2,020 properties were supported with the technologies, and 376 species of interest were identified. The RDAF and MNF had the greatest diversity of species group. AFS and RDAP concentrated on one main species, Theobroma cacao and Avena spp. respectively. Thus, it is found a listing of species with potential for projects with different low-carbon technologies in the Atlantic Forest, which are adapted to the local market and with economic and environmental benefits.
650 $aClimate change
650 $aRural development
650 $aSustainable development
650 $aDesenvolvimento Rural
650 $aMudança Climática
653 $aSustainability
700 1 $aASSAD, L. T.
700 1 $aOLIVEIRA NETO, S. N. de
700 1 $aBONET, M. S.
700 1 $aLEITE, F. F. G. D.
700 1 $aCANTO, A. C. B. do
700 1 $aCORDEIRO, F. R.
700 1 $aMUÑOZ, A. M.
700 1 $aRAMOS, M. B. de C.
700 1 $aRODRIGUES, R. de A. R.
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Registro original: |
Embrapa Solos (CNPS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
07/04/2010 |
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Data da última atualização: |
07/04/2010 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FARIAS, T. A.; ARAUJO, F. R.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA, I. I. F.; OLIVEIRA, R. H. M. de; AZAMBUJA, A.; ROSINHA, G. M. S.; SOARES, C. O.; ELISEI, C. |
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Afiliação: |
Thaís A. Farias, UFMS; FLABIO RIBEIRO ARAUJO, CNPGC; Ana Luíza A. R. Osório, UFMS; Kláudia S. G. Jorge, UFMS; Carlos A. N. Ramos, CNPGC; Ingrid I. F. Souza, CNPGC; RENATO HENRIQUE MARCAL DE OLIVEIRA, CNPGC; Alexandre Azambuja, UFMS; GRACIA MARIA SOARES ROSINHA, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; CARINA ELISEI, CNPGC. |
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Título: |
ELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. |
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Páginas: |
1 p. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease. MenosBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of... Mostrar Tudo |
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Thesagro: |
Diagnostico; Elisa; Mycobacterium Bovis; Sanidade Animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02453naa a2200301 a 4500
001 1663370
005 2010-04-07
008 2009 bl uuuu u00u1 u #d
100 1 $aFARIAS, T. A.
245 $aELISA based on recombinant MPB70 to detect antibodies to Mycobacterium bovis.
260 $c2009
300 $a1 p.$c1 CD-ROM.
520 $aBovine tuberculosis is an important infectious disease, caused by Mycobacterium bovis. The gene that encodes for MPB70 protein is present in the genome of M. bovis and has high specificity for the M. tuberculosis complex, representing a potential antigen for the diagnosis of bovine tuberculosis. The objective of this work was to develop an ELISA using recombinant protein MPB70 of M. bovis in order to detect antibodies against these bacteria in cattle. The gene mpb70 was amplified by PCR and cloned into plasmid pGEM-T Easy. After digestion with the Eco RI enzyme, the gene was subcloned into the expression plasmid pGEX-3X. The expression of the gene was verified by SDS-PAGE and Western blot with anti-glutathione-S-transferase. After standardization of the ELISA, serum samples of positive (53), negative (37) or inconclusive (47) cattle in the tuberculin test were tested. In SDS-PAGE, a recombinant protein of approximately 48 kDa was detected, which corresponds to the estimated molecular weight of GST/MPB70. In Western blot, recombinant MPB70, fused to GST, was recognized by anti-GST antibody, confirming the expression of the gene. For the ELISA with recombinant MPB70, the sensitivity and specificity found were 88.7% and 94.6%, respectively. The use of a serological test such as ELISA with recombinant MPB70, together with the cellular tests, may solve some of the problems found in the control programs of bovine tuberculosis, like inconclusive results and the lack of detection of anergic animals in advanced stages of the disease.
650 $aDiagnostico
650 $aElisa
650 $aMycobacterium Bovis
650 $aSanidade Animal
700 1 $aARAUJO, F. R.
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aSOUZA, I. I. F.
700 1 $aOLIVEIRA, R. H. M. de
700 1 $aAZAMBUJA, A.
700 1 $aROSINHA, G. M. S.
700 1 $aSOARES, C. O.
700 1 $aELISEI, C.
773 $tIn: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009.
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